Order	Organism	Abbr	EG#	Screen Condition	Reference
1	Acinetobacter baumannii ATCC 17978	acb	458	Rich medium	Wang, N., et al. (2014) Genome-wide identification of Acinetobacter baumannii genes necessary for persistence in the lung, MBio, 5, e01163-01114. [PubMed]
2	Acinetobacter baylyi ADP1	aci	499	Rich medium	de Berardinis V, et al (2008). A complete collection of single-gene deletion mutants of Acinetobacter baylyi ADP1. Mol Syst Biol. [PubMed]
3	Agrobacterium fabrum str. C58	atu	361	Rich medium	Curtis, P.D. and Brun, Y.V. (2014) Identification of essential alphaproteobacterial genes reveals operational variability in conserved developmental and cell cycle systems, Mol. Microbiol., 93, 713-735. [PubMed]
4	Bacillus subtilis 168	bsu	271	Rich medium	Kobayashi K, et al (2003). Essential Bacillus subtilis genes. Proc Natl Acad Sci U S A, 100:4678-83. [PubMed]
5	Bacteroides fragilis 638R	bfg	547	Rich medium.	Yaligara Veeranagouda, et al (2014). "Identification of genes required for the survival of B. fragilis using massive parallel sequencing of a saturated transposon mutant library". BMC Genomics, 15:429. [PubMed]
6	Brevundimonas subvibrioides ATCC 15264	bsb	412	Rich medium	Curtis, P.D. and Brun, Y.V. (2014) Identification of essential alphaproteobacterial genes reveals operational variability in conserved developmental and cell cycle systems, Mol. Microbiol., 93, 713-735. [PubMed]
7	Burkholderia pseudomallei K96243	bps	505	Rich medium	Moule, M.G., et al. (2014) Genome-Wide Saturation Mutagenesis of Burkholderia pseudomallei K96243 Predicts Essential Genes and Novel Targets for Antimicrobial Development. MBio 5, e00926-00913 [PubMed]
8	Burkholderia thailandensis E264	bte	406	Rich medium.	Baugh, Loren, et al. "Combining Functional and Structural Genomics to Sample the Essential Burkholderia Structome." PloS one 8.1 (2013): e53851. [PubMed]
9	Campylobacter jejuni subsp. jejuni NCTC 11168 = ATCC 700819	cje	228	Rich medium	Metris, Aline, et al. "In vivo and in silico determination of essential genes of Campylobacter jejuni." BMC genomics 12.1 (2011): 535. [PubMed]
10	Caulobacter crescentus	ccr	480	Rich medium	Christen B, et al (2011). The essential genome of a bacterium. Mol Syst Biol, 7:528. [PubMed]
11	Escherichia coli MG1655 II	eco	296	Rich medium	Baba T, et al (2006). Construction of Escherichia coli K-12 in-frame, single-gene knockout mutants: the Keio collection. Mol Syst Biol. [PubMed]
12	Francisella novicida U112	ftn	392	Rich medium	Gallagher LA, et al (2007). A comprehensive transposon mutant library of Francisella novicida, a bioweapon surrogate. Proc Natl Acad Sci U S A, 104:1009-14. [PubMed]
13	Haemophilus influenzae Rd KW20	hin	642	Rich medium	Akerley BJ, et al (2002). A genome-scale analysis for identification of genes required for growth or survival of Haemophilus influenzae. Proc Natl Acad Sci U S A, 99:966-71. [PubMed]
14	Mycoplasma genitalium G37	mge	381	Rich medium	Glass JI, et al (2006). Essential genes of a minimal bacterium. Proc Natl Acad Sci U S A, 103:425-30. [PubMed]
15	Mycoplasma pulmonis UAB CTIP	mpu	310	Rich medium	French CT, et al (2008). Large-scale transposon mutagenesis of Mycoplasma pulmonis. Mol Microbiol. [PubMed]
16	Porphyromonas gingivalis ATCC 33277	pgn	463	Rich medium	Klein BA, et al (2012). Identification of essential genes of the periodontal pathogen Porphyromonas. BMC Genomics, 13: 578. [PubMed]
17	Pseudomonas aeruginosa PAO1	pae	336	Rich medium	Turner, K.H., Wessel, A.K., Palmer, G.C., Murray, J.L. and Whiteley, M. (2015) Essential genome of Pseudomonas aeruginosa in cystic fibrosis sputum. Proc. Natl. Acad. Sci. U. S. A. [PubMed]
18	Rhodopseudomonas palustris CGA009	rpa	522	Rich medium	Pechter, Kieran B., et al. "The essential genome of the metabolically versatile alphaproteobacterium Rhodopseudomonas palustris." Journal of bacteriology (2015): JB-00771. [PubMed]
19	Salmonella enterica serovar Typhi Ty2	stt	358	Rich medium	Barquist, Lars, et al. "A comparison of dense transposon insertion libraries in the Salmonella serovars Typhi and Typhimurium." Nucleic acids research 41.8 (2013): 4549-4564. [PubMed]
20	Salmonella enterica serovar Typhimurium SL1344	sey	353	Rich medium	Barquist, Lars, et al. "A comparison of dense transposon insertion libraries in the Salmonella serovars Typhi and Typhimurium." Nucleic acids research 41.8 (2013): 4549-4564. [PubMed]
21	Salmonella typhimurium LT2	stm	230	Rich medium	Knuth K, et al (2004). Large-scale identification of essential Salmonella genes by trapping lethal insertions. Mol Microbiol, 51:1729-44. [PubMed]
22	Shewanella oneidensis MR-1	son	403	Rich medium	Deutschbauer, Adam, et al. "Evidence-based annotation of gene function in Shewanella oneidensis MR-1 using genome-wide fitness profiling across 121 conditions." PLoS genetics 7.11 (2011): e1002385. [PubMed]
23	Sphingomonas wittichii RW1	swi	535	Rich medium	Roggo, Clemence, et al. "Genome-wide transposon insertion scanning of environmental survival functions in the polycyclic aromatic hydrocarbon degrading bacterium Sphingomonas wittichii RW1." Environmental microbiology (2013). [PubMed]
24	Staphylococcus aureus NCTC 8325	sao	351	Rich medium	Chaudhuri RR, et al (2009). Comprehensive identification of essential Staphylococcus aureus genes using Transposon-Mediated Differential Hybridisation (TMDH). BMC Genomics, 10:291. [PubMed]
25	Streptococcus agalactiae A909	sak	317	Rich medium	Hooven, T.A. et al. The Essential Genome of Streptococcus Agalactiae.BMC Genomics, 17, 406, 2016. [PubMed]
26	Streptococcus pyogenes MGAS5448	spz	227	Todd-Hewitt medium	Le Breton Y. et al (2015). Essential Genes in the Core Genome of the Human Pathogen Streptococcus pyogenes. Sci. Rep. 5, 9838. [PubMed]
27	Streptococcus pyogenes NZ131	soz	241	Todd-Hewitt medium	Le Breton Y. et al (2015). Essential Genes in the Core Genome of the Human Pathogen Streptococcus pyogenes. Sci. Rep. 5, 9838. [PubMed]
28	Streptococcus sanguinis	ssa	218	Rich medium	Xu, P et al(2011). Genome-wide essential gene identification in Streptococcus sanguinis. Sci. Rep. 1, 125; DOI:10.1038/srep00125. [PubMed]
29	Synechococcus elongatus PCC 7942	syf	682	Rich medium	Rubin, Benjamin E., et al. "The essential gene set of a photosynthetic organism." Proceedings of the National Academy of Sciences (2015): 201519220. [PubMed]
30	Vibrio cholerae N16961	vch	779	Rich medium	Cameron DE, Urbach MJ,Mekalanos JJ(2008). A defined transposon mutant library and its use in identifying motility genes in Vibrio cholerae. PNAS, 105(25):8736-8741. [PubMed]